Abstract
Apigenin is one of the primary flavonoids in celery, which has a high medicinal value. Flavone synthase I (FNSI) is the last step enzyme in apigenin biosynthesis. In this study, the 1492 bp promoter sequence before AgFNSI initiation codon (ATG) of celery was obtained, which included methyl jasmonate (MeJA) responsive elements, light responsive elements, anaerobic induction elements and five MYB binding sites. AgFNSI was sensitive to temperature, UV-B, water deficit and MeJA. Comparative analysis of AgFNSI genome and promoter sequences among celery accessions with different apigenin content showed that there were four allelic variations in AgFNSI, and four accessions with high apigenin content belonged to AgFNSIa, and five accessions with low apigenin content belonged to AgFNSIc. Three pairs of dominant complementary markers were designed based on the single-nucleotile polymorphisms (SNPs) of the AgFNSIa and AgFNSIc genomes and promoter sequences. Three pairs of functional markers were validated by 112 celery accessions. The results showed that AFPA1/AFPB1 detected significant differences in apigenin content between different genotypes. Therefore, marker AFPA1/AFPB1 is associated with apigenin content in celery and could be used for the genetic improvement of apigenin content in celery.
Highlights
Improvements in living standards have fostered increased awareness of dietary healthy
The results showed that both high- and low-temperature treatments could affect the expression of AgFNSI
The results showed that AgFNSI was sensitive to temperature, UV-B, water deficit and methyl jasmonate (MeJA), and long-term treatment could affect the apigenin content
Summary
Improvements in living standards have fostered increased awareness of dietary healthy. The expression pattern of FNSI in celery under environmental stress and plant hormone treatments and its relationship with apigenin accumulation have not been reported. Functional markers are recent development based on the phenotypic differences caused by the polymorphic sequences of target genes and are able to determine the existence of target alleles in a variety of genetic backgrounds[13]. Exploring the characteristics of the FNSI gene and developing corresponding functional markers should improve the accuracy of phenotypic prediction and the efficiency of molecular -assisted breeding, which is of great significance for improving celery quality. Real-time fluorescence quantitative PCR (qRT-PCR) was used to analyze the expression patterns of AgFNSI in celery under different environmental stress and plant hormone treatments. Functional markers were developed to detect the allelic variation associated with apigenin accumulation in celery
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