Express of the nerve growth factor of the injuried spinal cord after olfactory ensheathing cells transplantation

Abstract

Objective In order to approach the mechanism of the spinal cord injury (SCI) repaired by OECs transplantation,the effects of olfactory ensheathing cells (OECs) transplantation on the expression of the nerve growth factor (NGF) in injured spinal cord was quantitatively studied.Methods The rat models of SCI were made by NTY - Ⅱ impactor ( 10g -25 mm) at T10 level There are 6 rats in the normal group and 48 rats in the injuried group. Then the injuried group was randomly divided into OECs transplantation group and DMEM group.The GFP - OECs were transplanted into the injured region immediately after SCI in the OECs transplantation group.The DMEM group used the DMEM/F12 to take the place of GFP - OECs.Nothing to do with normal control Thcn the locomotor function was appraised by BBB score at 1 day,7 day,14 day and 21 day.The expression of the NGF in the injured region was detected by the methods of immunohistochemistry and RT - PCR.Results The BBB score in OECs transplantation group was higher than the DMEM group and normal group at 1 day,7 day,14 day and 21 day.The expression of the NGF was advanced at 1 day,and highest at 7 day.The expression of the NGF in the OECs group was higher than the normal control group and the DMEM group at 21 day.Conclusions The expression of NGF can be up - regulated by OECs transplantation,which may be one of the mechanisms for OECs repairing injured spinal cord. Key words: Spinal cord injury; Olfactory ensheathing cells; Cell transplantation; Nerve growth factor