Expresión de CCL-7 y CCR-5 en Lesiones Periapicales

Abstract

IntroductionOne of the key phenomena in Apical Lesions (AL) pathogenesis is chemotaxis. CCL-7 is a very potent chemoattractant for monocytes, and CCR-5 corresponds to one of its receptors. Until now, CCL-7 and CCR-5 expression in AL remains unknown. ObjectivesTo study the expression of CCL7 and CCR-5 in AL. MethodsPeriapical exudates (PE) samples were collected from root canals during routine endodontic treatments from teeth with diagnosis of symptomatic (n=15) and asymptomatic Apical Periodontitis (AP) (n=15), and “immunowestern blot” was conducted to assess the expression of CCL-7. The intensity of the bands was measured by densitometric analysis. As controls, homogenized tissue from healthy periapical ligaments was obtained during teeth extraction for orthodontic reasons (n=10). To determine the expression of CCL-7 and CCR-5 in AL, biopsies from teeth with clinical diagnosis of chronic AP were obtained during apical surgery procedures, processed by routine technique and histopathologically diagnosed as Apical Granuloma (AG) (n=8) or Radicular Cyst (RC) (n=2) and then, analyzed by immunohistochemistry. The samples were examined with light microscope. As controls, biopsies from healthy periapical ligaments obtained during teeth extraction for orthodontic reasons were used. ResultsCCL-7 was highly expressed in PE samples, and its expression could not be observed in healthy controls. The presence of CCL-7 and CCR-5 in AG and RC is mainly located to the inflammatory infiltrate. No expression was observed in healthy controls. ConclusionsCCL-7 and CCR-5 may play a role in AP pathogenesis, may be by stimulating chemotaxis.