Exposure to silver impairs the osmoregulatory capability of euryhaline medaka (Oryzias latipes) subjected to salinity changes

Abstract

The widespread use of silver in nanomaterials has led to increases in environmental contamination, which poses a threat to aquatic animals. Euryhaline fish, which live in environments with fluctuating salinity levels, have strong osmotic regulatory abilities to cope with such changes. This study attempted to investigate how silver affects the osmoregulatory capabilities of euryhaline fish, using medaka (Oryzias latipes) embryos as a model. The embryos were exposed to AgNO3 for 7 d in either fresh water (FW) or seawater (SW), and their mortality, heart rate, morphology, and ionocytes were examined. Results showed that the toxicity of AgNO3 was higher in FW than in SW (50% lethal concentrations (LC50) were 0.17 vs. 1.01 ppm). Although AgNO3 (0.05 and 0.1 ppm) did not significantly change the morphology of embryos, it impaired ionocytes and elevated heart rates in FW. While, AgNO3 (0.1 and 0.5 ppm) did not affect the morphology, ionocytes, or heart rate in SW, it impaired the hypo-osmoregulatory capability and elevated the mortality of embryos that were transferred from FW to SW. At 12 h after SW transfer, ionocytes were severely impaired, and water-drinking behavior was suppressed, resulting in body dehydration and sodium overload. In contrast, AgNO3 did not elevate the mortality of embryos that were transferred from SW to FW. To sum up, the presence of silver in FW during the developmental stage of euryhaline fish could potentially endanger their survival during SW adaptation.