Exposure to low or high light intensity affects pituitary-testicular activity in the fish Oreochromis mossambicus

Abstract

Light is an important factor for the successful reproduction of most fish. In this investigation, effect of different light intensities on pituitary-testis axis was studied for a period of 21 days, under normal photoperiodic regime in the tilapia Oreochromis mossambicus. The mean numbers of spermatogonia (Sg), primary spermatocytes (Ps), secondary spermatocytes (Ss), early spermatids (Est) and late spermatids (Lst) did not show significant difference between fish exposed to moderate light intensity (MLI) and initial controls or controls, whereas the mean numbers of Sg were significantly lower in fish exposed to low light intensity (LLI) compared to those of initial controls, controls and MLI groups. However, the mean numbers of Ps, Ss, Est and Lst were significantly lower in fish exposed to LLI and high light intensity (HLI) compared to those of other experimental groups. Furthermore, in the pituitary gland, weakly immunoreactive luteinizing hormone (LH) secreting cells were observed in the proximal pars distalis (PPD) region in fish exposed to LLI and HLI in contrast to the intense immunolabelling of these cells in initial controls, controls and MLI groups. The androgen receptors showed diminished immunoreactivity in the Sertoli cells along the seminiferous lobules of the testis in fish exposed to LLI and HLI, whereas the strongly immunoreactive androgen receptors were observed in the Sertoli cells in initial controls, controls and MLI groups. Taken together, these results indicate that long-term exposure to low or high light intensity light suppresses spermatogenetic process and that this inhibition is due to reduced secretory activity of LH cells in the pituitary gland and androgen secretion in the testis of the fish O. mossambicus.