Abstract
Environmental pollutants, such as bisphenol A (BPA) have recently been implicated in the development of adverse birth outcomes. However, the underlying teratogenic mechanisms remain unclear. We investigated the effects of BPA on the migration and invasion of human primary extravillous trophoblast HTR-8/SVneo cells. Our results indicated that BPA reduced cell migration and invasion. Moreover, it altered the ratio of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) by downregulating MMP-2 and MMP-9, and upregulating TIMP-1 and TIMP-2. Furthermore, BPA suppressed integrin β1, integrin α5, and vimentin. Interestingly, BPA-induced invasion was partially restored by G15, a membrane G-protein-coupled estrogen receptor 30 antagonist. We further revealed that 42 proteins were differentially expressed by mass spectrometry analysis, which could be divided into three categories based on gene ontology including biological process, cellular component, and molecular function. These results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and MMP-9, up-regulating TIMP-1 and TIMP-2, and suppressing adhesion molecules.
Highlights
Bisphenol A (BPA) is an environmental pollutant and an endocrine system modulator commonly used in the synthesis of polycarbonate plastics
These results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating matrix metalloproteinases (MMPs)-2 and MMP-9, up-regulating tissue inhibitors of MMPs (TIMPs)-1 and TIMP-2, and suppressing adhesion molecules
Our results suggest a potential mechanism by which BPA plays a role in the regulation of cell migration and invasion in human primary extravillous trophoblast HTR-8/SVneo cells
Summary
Bisphenol A (BPA) is an environmental pollutant and an endocrine system modulator commonly used in the synthesis of polycarbonate plastics. Studies on BPA in body fluids, Received 17 January 2020, Revised 04 May 2020, Accepted 20 May 2020, Epub 30 June 2020 CLC number: R114, Document code: A The authors reported no conflict of interests. The mechanisms underlying BPA-induced toxicity during embryo implantation remain unclear
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