Exposure to different wavelengths of light and the development of structural and functional asymmetries in the chicken

Abstract

The thalamofugal visual projections of the chick are known to develop in response to stimulation by light prior to hatching, and asymmetry in the number of projections develops as a consequence of the embryo being oriented in the egg so that it occludes its left eye. The right eye only is stimulated by light and this causes the visual projections connected to the right eye to develop in advance of those connected to the left. We have now found that exposure of embryos, from day 19 of incubation to hatching, to red (peak transmission at 670 run) or green (peak at 500 nm) light is as effective as broad-spectrum (white) light in establishing asymmetry in these projections. The intensities of the light to which the embryos were exposed in each case were equivalent, achieved in part by removing the air sac end of the egg shell. The thalamofugal visual projections, therefore, develop in response to light stimulation but appear to have no wavelength specificity. This result is consistent with the apparent lack of involvement of the thalamofugal visual pathway in colour vision. However, functional asymmetry, tested as left-right eye differences in categorising grain from pebbles, was found to be less marked in the chicks that had been exposed to green light compared to those that had been exposed to ‘white’ light, and it was absent in those exposed to red light or incubated in the dark. Thus, there is wavelength specificity for the development of the behavioural asymmetry, which suggests involvement of colour-coded neurons outside the thalamofugal visual pathway, probably in the tectofugal pathway. Exposure of the embryos to red and green light alternating at 30 min intervals is as effective as ‘white’ light for establishing both the structural and functional asymmetry.