Exposure of male tilapia (Oreochromis niloticus) to copper by intraperitoneal injection: DNA damage and larval impairment

Abstract

The present work aimed to assess the genotoxic effects of intraperitoneally injected copper in males of O. niloticus, and to investigate its association with eventual changes in the quality of the gametes and in the generation of offspring abnormalities. Thus, to evaluate DNA damage, the alkaline comet assay was performed in sperm and blood; sperm parameters were analyzed to determine the quality of the gametes, and the incidence of morphological abnormalities of larvae, originated from eggs fertilized by males exposed to copper, was used as a measure of reproductive impairment. Male fish were exposed to three doses (1 mg/kg, 2 mg/kg, 4 mg/kg) of copper sulfate via intraperitoneal injection and observed after 96 h of exposure. We found that the quality of gametes was not affected by copper, as indicated by the lack of differences in sperm parameters between the exposed and the control groups. Nonetheless, copper induced an increase in sperm DNA damage at all tested doses. Taking into consideration the total number of abnormalities, larvae presented a higher proportion of morphological abnormalities at all the tested doses. Considering the morphological abnormalities separately, the 2 mg/kg and 4 mg/kg groups presented significantly higher frequencies of arrested development and spinal abnormalities than the control group. This work suggests that intraperitoneally injected copper is an inductor of DNA breakage in germ cells of O. niloticus males. Copper induces the formation of larval morphological abnormalities, causing reproductive impairment to this species. The fact that copper did not induce alterations on sperm parameters indicates that the parental genotoxic effects are inheritable to the offspring.