Abstract
Abstract Magnetic field (MF) regulates several biological processes in living organisms including seed germination. This study was aimed to know the reason for rapid germination of MF pretreated soybean seeds by analyzing enzymatic changes in cotyledons and embryonic-axis. The fresh weight of cotyledons and embryonic-axis was gradually increased from 2 to 10 days, and their greater enhancement was observed in initial days of MF treated soybean. The activity of α-amylase was accelerated and nitrate-reductase was suppressed in both organs of seedlings exposed with MF. Acid and alkaline-phosphatase activities were initially decreased and later increased in embryonic-axis of MF treated seedlings, and nevertheless acid-phosphatase expression in MF cotyledons was declined. Hence, this is the first report of MF induced enzyme changes in germinating seeds, and the results of present study revealed that MF exposure regulates the expression of different enzymes, which play vital role to stimulate the rapid germination of soybean seeds.
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