Abstract
BackgroundAedes aegypti is a major insect vector because it transmits dreadful viruses as adults that cause disease in humans and other vertebrates. The use of mosquito’s microbiota has shown great potential impacts on vector control and mosquito reproductive competence. The present study aimed to examine the resident bacteria of mosquitoes which are used as a potent range to reduce the A. aegypti fitness. Isolated resident-bacterial strains from blood-fed Aedes species were characterized using gene sequencing and phylogenetic analysis, to assess the inhabitant bacterial strains survival rate in A. aegypti midgut, instar developmental duration, malformation and reproductive competence.ResultsThe genetic distinctiveness of isolated bacterial strains belong to the genus Exiguobacterium spp. and further non-redundant nucleotide database search revealed that the species of effective strains were E. aestuarii (MN629357) and E. profundum (MN625885). Exposure of the freshly hatched larvae with these bacteria cell densities extended the developmental duration. For instance, exposure of A. aegypti larva with 0.42 × 108, 0.84 × 108 and 1.68 × 108 cells/mL of E. aestuarii extended the total developmental duration to 11.41, 14.29 and 14.78 days, respectively. It also reduced the fecundity and hatchability of A. aegypti female, with exposure to these bacteria, from 1033.33 eggs/10 females in the control series to 656.67 eggs/10 females.ConclusionsThese present findings indicate that the resident-bacterial strains from blood-fed mosquito not only extend the larval durations but also rendered the A. aegypti females sterile to various extents.
Highlights
Aedes aegypti is a major insect vector because it transmits dreadful viruses as adults that cause disease in humans and other vertebrates
In the present study, bacterial diversity in the midgut from blood-fed mosquito species was analyzed by using pure culture methods
Characterization of gut bacteria in the mosquito collected from all four sites using a culture-dependent method led to the identification of 41 bacterial isolates and the number of colonies was increased with increase in incubation duration
Summary
Aedes aegypti is a major insect vector because it transmits dreadful viruses as adults that cause disease in humans and other vertebrates. For reducing the vector competence of mosquito, the approach of microbial symbionts plays a novel method to control the spread of arthropod-transmitted pathogens (Cirimotich et al, 2011) and various aspects of genetic manipulated Wolbachia and its role in the suppression of mosquito’s population reviewed (Mishra et al, 2018). The present study has been focused an overview of the diversity of resident-bacterial strains from blood-fed Aedes spp. their potential functions in mosquito reproductive biology and future applications in resident-based mosquito control strategies or in order to reduce the mosquito competence
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