Exportin-1 is required for the maintenance of the planarian epidermal lineage

Abstract

Nucleocytoplasmic transport is essential for normal cellular function that mediates cargo transport from the cytoplasm to the nucleus. However, the mechanisms of nucleocytoplasmic transport that integrate stem cell development remain largely unknown. Since it has a large population of stem cells, the planarian flatworm is an ideal system for the study of adult stem cell lineage development in vivo. Here, we focus on exportin-1, which is the most conserved nuclear export receptor. Homologs of exportin-1 have no currently known role in stem cell biology. RNA interference targeting exportin-1 caused a failure in anterior and posterior regeneration, and resulted in curly and lysis phenotypes in both intact and regenerating flatworms. During the course of exportin-1 RNAi phenotype, cell division was significantly decreased, and the expression of the epidermal cell markers (vimentin and laminB) were lost from the intact body. Additionally, the expression levels of the neoblast marker piwiA decreased. By contrast, the expression levels of the epidermal progenitor markers NB21.11e and AGAT1 increased. These results suggest that exportin-1 is required for the maintenance of the epidermal lineage in planarians. Inhibition of exportin-1 could promote the premature differentiation of neoblasts to the epidermal lineages, disrupting the proper epidermal maturation.