Exploring the Therapeutic Potential of Sedum lineare Thunb: Phytochemical Analysis and Identification of Active Bioactive Compounds

Abstract

Objectives: This research aimed to explore the therapeutic potential of Sedum lineare Thunb by conducting phytochemical analysis and identifying active bioactive compounds. Materials and Methods: S. lineare Thunb samples were collected from Pune district, Maharashtra, India in December 2022. Preparation of extracts using a hydroalcoholic solvent (ethanol: water, 70:30 v/v). Phytochemical screening using established methods to determine the presence of alkaloids, flavonoids, glycosides, diterpenes, carbohydrates, saponins, and tannins. Quantitative determination of total phenol and flavonoid contents in the hydroalcoholic extract. Identification of the marker compound (quercetin) in the S. lineare Thunb extract using HPLC. Results and Discussion: Alkaloids, glycosides, flavonoids, diterpenes, carbohydrates, saponins, and tannins were all found in the crude extracts by phytochemical analysis. The hydroalcoholic extract exhibited total phenol and flavonoid contents of 2.75 and 1.452 mg/100 mg, respectively. Quantitative estimation of quercetin in the hydroalcoholic extract was determined to be 0.0148%. Conclusion: The study demonstrated that S. lineare Thunb extracts contain various secondary metabolites, including phenolic compounds, flavonoids, and other bioactive compounds. The hydroalcoholic extract showed significant total phenol and flavonoid contents, indicating its potential therapeutic value. Further research is warranted to explore the specific health benefits and therapeutic applications of the identified bioactive compounds in S. lineare Thunb