Abstract
Temperature is a crucial parameter for biological and chemical processes. Its effect on enzymatically catalysed reactions has been known for decades, and stereo‐ and enantiopreference are often temperature‐dependent. For the first time, we present the temperature effect on the Baeyer‐Villiger oxidation of rac‐bicyclo[3.2.0]hept‐2‐en‐6‐one by the type II Bayer‐Villiger monooxygenase, 2,5‐DKCMO. In the absence of a reductase and driven by the hydride‐donation of a synthetic nicotinamide analogue, the clear trend for a decreasing enantioselectivity at higher temperatures was observed. “Traditional” approaches such as the determination of the enantiomeric ratio (E) appeared unsuitable due to the complexity of the system. To quantify the trend, we chose to use the ‘Shape Language Modelling’ (SLM), a tool that allows the reaction to be described at all points in a shape prescriptive manner. Thus, without knowing the equation of the reaction, the substrate ee can be estimated that at any conversion.
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