Abstract
A method combining solid-phase extraction (SPE) on silica (Si) and aminopropyl-bonded silica (NH2) columns has been applied for the separation of phospholipids (PL), glycolipids (GL), neutral lipids (NL) and triglyceride fatty acids from the spores of Rhizophagus irregularis DAOM 197198 and in olive rhizosphere with and without arbuscular mycorrhizal (AM) fungi. Chromatography on the Si-column resulted in the separation of NL, GL and PL. Chromatography on the NH2- column led to the separation of TG from NL, previously coeluted on the Si-column. The fatty acid composition of TG of Rhizophagus irregularis spores obtained using this method showed higher amounts of 16:1ω5 (40%). The TGFA 16:1ω5 was present in higher levels in rhizosphere with AM fungi than in rhizosphere without AM fungi. This Si/NH2 method provided a rapid separation of TGFA 16:1ω5, commonly found in living cells, and will be useful to quantify the biomass of AM fungi in soil.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
