Abstract
Cerebrovascular disease is the main cause of death in the world. Here, we explored whether circulating serum miR-148b-3p, miR-151b and miR-27b-3p could be as potential diagnostic biomarkers for diagnosing acute ischemic stroke. Seventy-seven IS patients and forty-two healthy controls matched for age and sex were enrolled in the present study. Blood samples were drawn from IS patients within the 24 h. The correlation analysis was performed by Spearman. The ability to distinguish patients from healthy controls was determined by receiver operating characteristic (ROC) curve. The expression of circulating serum miR-148b-3p was significantly decreased, whereas miR-151b and miR-27b-3p were elevated significantly compared with controls. ROC analysis showed area under the ROC curve (AUC) of miR-148b-3p, miR-151b and miR-27b-3p to be 0.6647, 0.6852 and 0.6657, respectively. While the AUC increased to 0.8103 for the combination of miR-148b-3p and miR-27b-3p. Blood miR-151b level was negatively correlated with insulin-like growth factor-1 (IGF-1), and miR-27b-3p level was negatively correlated with IGF-1 and insulin-like growth factor binding protein-3, respectively. Our findings suggest that miR-148b-3p, miR-151b and miR-27b-3p may serve as blood-based biomarkers for diagnosing ischemic stroke patients, and the combination of miR-148b-3p and miR-27b-3p may be more powerful.
Highlights
Cardiovascular and cerebrovascular disease became the two leading cause of years of life lost, and in China, the leading cause of death in 2016 was cerebrovascular disease for men and women [1]
The diagnosis today mainly based on clinical examinations, confirmed with magnetic resonance imaging (MRI) or computed tomography (CT), which are time-consuming and expensive
There was positive correlation between serum miR-148b level and glucose (r = −0.2856, P=0.0422) (Figure 2A), miR-151b level showed a strong negative correlation with insulin-like growth factor-1 (IGF-1) (r = −0.4606, P=0.0004) (Figure 2B), and miR-27b-3p level was negatively correlated with IGF-1 (r = −0.3193, P=0.0165) (Figure 2C) and insulin-like growth factor binding protein-3 (IGFBP-3) (r = −0.3015, P=0.0267) (Figure 2D), respectively
Summary
Cardiovascular and cerebrovascular disease became the two leading cause of years of life lost, and in China, the leading cause of death in 2016 was cerebrovascular disease for men and women [1]. Until recently, there are still no current blood biomarkers to predict and diagnose ischemic stroke. It is urgent to develop serum/plasma biomarkers for accurate prediction and diagnosis of ischemic stroke. MicroRNAs (miRNAs or miRs) are a group of small (19–23 nt) non-coding single strand RNA molecules that play endogenous roles in regulating gene expression through binding to 3’-untranslated regions of target mRNAs [2]. Lots of reports have demonstrated that specific circulating c 2018 The Author(s).
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