Abstract
Efficient application of veterinary blood transfusion approaches for small companion animals requires readily available supply of the donor material. This can be achieved by developing of effective biobanking technologies allowing long-term storage of donor blood components via cryopreservation. Transfusion of an erythrocyte concentrate allows the successful correction of various hematological pathologies, severe bleeding, and etc. While in the past there were several approaches to cryopreserve red blood cells of dogs, to our knowledge there is virtually no data on cryopreservation of feline erythrocytes. In this paper, we performed a comprehensive parameter optimization for low temperature storage of RBCs of both species. Here, the efficiency of single-component and multicomponent cryoprotective media as well as necessary time of pre-incubation with penetrating and non-penetrating cryoprotectants prior to rapid freezing is analyzed. This study showed that glycerol was not sufficient for cryopreservation of red blood cells of the studied species under the investigated conditions. Application of 10% (v/v) ME2SO allowed for a significant reduction of canine and feline erythrocytes hemolysis after thawing. 17.5% hydroxyethyl starch demonstrated the highest cryoprotective activity for both species. It was found that dog RBCs should be incubated in cryoprotective media for 30 min at 22°C prior to freezing, while for cat RBCs 20 min is sufficient. Combination of CPAs was less effective. Presented data may be considered in further studies in veterinary transfusion and blood banking optimization.
Highlights
Development of methods for low-temperature preservation and long-term storage of red blood cells (RBC) of domestic animals, dogs and cats, is one of the actual problems in veterinary medicine [1,2,3]
Erythrocytes of cats and dogs from the control group were exposed in isotonic saline solutions for up to 30 min after preparation without any further impact factors
Survival of erythrocytes of both species was higher than 99%
Summary
Development of methods for low-temperature preservation and long-term storage of red blood cells (RBC) of domestic animals, dogs and cats, is one of the actual problems in veterinary medicine [1,2,3]. The advantage of erythrocyte concentrate transfusions, in comparison with the whole blood, is the standardized amount of contained erythrocytes and hemoglobin along with minimal presence of citrate, leukocytes, platelets, and degradation products [8,9]. We were interested on the comparison between the possibility for cryopreserving red blood cells of different animal species taking into consideration their peculiarities. Red blood cells of dogs and cats have a slight variation in size with an average volume of 41–51 μm and 62–74 μm, respectively [13]. The content of hemoglobin (90-170 g/l for cats and 120–170 g/l for dog) and its structure differs among these species [15,16]
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