Exploring the Association of Innate Immunity Biomarkers With MRI Features in Both Early and Late Stages Osteoarthritis.

Sureka Naidu Rajandran,Cheryl Ann Ma,Jin Rong Tan,Jin Liu,Steven Bak Siew Wong,Ying-Ying Leung

doi:10.3389/fmed.2020.554669

Sureka Naidu Rajandran, Cheryl Ann Ma + Show 4 more

Open Access

https://doi.org/10.3389/fmed.2020.554669

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Abstract

Objective: To evaluate the association between biomarkers of innate immunity and the magnetic resonance imaging (MRI) features of earlier and later stages of knee osteoarthritis (KOA).Methods: From 139 and 20 participants with earlier and later stages of KOA, respectively, we analyzed knee MRIs scored using the Boston Leeds Osteoarthritis Knee Score (BLOKS) at recruitment with biomarkers. In paired serum (s) and synovial fluid (sf), we quantified three biomarkers related to innate immunity: lipopolysaccharide binding protein (LBP), CD14 and Toll-like receptor 4 (TLR4), and three proinflammatory biomarkers [interleukin-6 (IL6), IL8, and tumor necrosis factor alpha (TNFα)].Results: In participants with earlier KOA, (s) LBP was statistically significantly associated with meniscal extrusion, and (sf) CD14 was associated with effusion after adjustment with age, sex, and body mass index. In participants with later stage of KOA, (sf) LBP was associated with effusion. (sf) CD14 was associated with cartilage loss and BML. In earlier stage of KOA, the proinflammatory biomarkers IL6, IL8, and TNFα were associated with most MRI features.Conclusion: Innate immunity biomarkers (s) LBP was associated with MRI meniscal extrusion; (sf) CD14 was associated with MRI synovial inflammation in earlier stage and BMLs in later stage of KOA. Associations between proinflammatory biomarkers and various MRI features in earlier stage of KOA were observed.

Highlights

Knee osteoarthritis (KOA) is the most common form of arthritis affecting about 250 million people worldwide and a leading cause of mobility and disability in the elderly [1, 2]
We aim to evaluate the association of inflammatory biomarkers related to innate immunity: lipopolysaccharide binding protein (LBP), cluster of differentiation 14 (CD14), Toll-like receptor 4 (TLR4) with different Magnetic resonance imagining (MRI) features of the knee from two studies of participants with KOA
We evaluated the associations between the (s) and biomarkers with MRI features, Kellgren and Lawrence (KL) gradings, and WOMAC scoring using generalized linear models adjusted for age, sex, and body mass index (BMI)

Summary

Introduction

Knee osteoarthritis (KOA) is the most common form of arthritis affecting about 250 million people worldwide and a leading cause of mobility and disability in the elderly [1, 2]. Inflammation of the synovium may play a role in the pathophysiology of KOA, as evidenced by histological synovitis from the tissue harvested from patients undergoing arthroplasties [3,4,5]. There is less information on earlier stages of KOA, and the pathophysiological characteristics of earlier. Greater knowledge of the initial stages of the disease may help advance understanding of KOA pathogenesis and identification of therapeutic targets for early KOA, which may be more responsive to treatment. Magnetic resonance imagining (MRI) is an imaging modality that reveals the pathology of all joint tissues including the cartilage, synovium, meniscus, and bone marrow. MRI can detect synovial membrane inflammation in early KOA even when signs of joint inflammation were not obvious in physical examination [6, 7]; it provides a mean for studying earlier stages of KOA

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