Abstract
BackgroundPao zhi is a common traditional approach that usually occurs before most herbs are prescribed whereby during processing, secondary plant metabolites are transformed, thus helping to increase potency, reduce toxicity and altering their effects. Using Radix Aconiti (Chuan Wu, CW) as a model herb, suitable chemical markers are crucial for studying the processing mechanisms of these herbs.ResultsIn this study, the comprehensive metabolomic characters of CW and Prepared CW (ZCW) by RPLC-Q-TOF/MS were investigated to guarantee clinical safety. Multivariate analyses successfully identified specific metabolite changes between CW and ZCW. In addition, 22 key biomarkers responsible for the detoxifying actions of pao zhi were discovered. The processing mechanism of CW were discussed according to the identified metabolites. This method is efficient, providing more accurate characterisations of traditional pao zhi detoxification.ConclusionsThe proposed strategy proves that RPLC-Q-TOF/MS-based metabolomic analysis does not only explore chemical markers but can also provide a comprehensive understanding of the transformation mechanisms underlying pao zhi.
Highlights
Pao zhi is a common traditional approach that usually occurs before most herbs are prescribed whereby during processing, secondary plant metabolites are transformed, helping to increase potency, reduce toxicity and altering their effects
The underlying regulations of pao zhi perturbed metabolic pathways were discussed and the processing mechanism of Chuan Wu (CW) was elucidated according to the results of chemical markers for CW and Prepared CW (ZCW)
This novel method can be valuable for rapidly exploring potential chemical markers and studying processing mechanisms of herbs
Summary
The comprehensive metabolomic characters of CW and Prepared CW (ZCW) by RPLC-Q-TOF/MS were investigated to guarantee clinical safety. Multivariate analyses successfully identified specific metabolite changes between CW and ZCW. 22 key biomarkers responsible for the detoxifying actions of pao zhi were discovered. The processing mechanism of CW were discussed according to the identified metabolites. This method is efficient, providing more accurate characterisations of traditional pao zhi detoxification
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