Exploring Phytochemical profiling, Antioxidant, Enzyme Inhibition, and Antibacterial Potential of Callistemon viminalis (Sol. ex Gaertn.) G.Don ex Loudon)by In vitro and In silico Approaches.

Abstract

Callistemon viminalis (Sol. ex Gaertn.) G. Don ex Loudon) (Family: Myrtaceae) is used for its medicinal properties in treating various metabolic disorders. We investigated the chemical characterization and biological screening of n-hexane extract of C. viminalis. Total phenolic content was (37.45 ± 7.40 mg GA.E/g D.E ± S.D) and total flavonoid content was (18.43 ± 6.34 mg R.E/g D.E ± S.D). The GC-MS screening of n-hexane extract tentatively identified 70 bioactive phytochemicals. The maximum antioxidant potential (289.99 ± 9.01 mg T.E/g D.E±S.D) was observed by FRAP assay. The enzyme inhibition assays revealed α-glucosidase and α-amylase inhibition (6.9 ± 0.13 and 7.2 ± 0.56 mmol of ACA.E/g D.E±S.D), urease (4.95 ± 0.9 mg of TU.E/g D.E±S.D), acetylcholinesterase (2.9 ± 0.08 mg GALA.E/g D.E±S.D), lipoxygenase (4.93 ± 1.05 mg of Indo.E/g D.E±S.D) and tyrosinase (4.33 ± 0.62 mg of KA.E/g D.E±S.D) inhibition. The extract showed antibacterial potential against Salmonella typhi (53.90±5.05)Bacillus subtilis (68.55±2.70%), Salmonella aureus (71.30±4.44%) and Pseudomonas aeruginosa (57.86±6.02%). The docking studies revealed a good docking interaction of ligands against the studied enzymes while ADME analysis revelaed pharmacokinetic profile of thephytoconstituents.C. viminalispossesses promising therapeutic potential and can be further explored for drug development and drug designing.