Exploring Mixed Hydrotropy Concept in HPTLC Densitometric Estimation of Telmisartan.

Abstract

Analysis of the poorly water-soluble drugs most frequently carried out by using organic solvents like methanol, ethanol, chloroform, acetonitrile, and hexane. Most of them have toxic effects on humans as well as the natural ecosystem. The volatile nature of organic solvents is a major source of inaccuracy. So, the current study provides the best approach to replace organic solvents as hydrotropic solubilization techniques and developed a green, eco-friendly method to estimate telmisartan by highperformance thin-layer chromatography (HPTLC). Using silica gel coated (60 F254) TLC plates, chromatographic separation was achieved with a mobile phase 30% Sodium Benzoate (SB): 20% sodium acetate (SA): 0.5% citric acid (CA) (6:3:0.5 v/v/v). The retention factor for telmisartan was 0.55. Concentrations were linear in between 500 to 900 µg/mL with a regression coefficient was 0.9930. This approach was successfully validated as per the International Conference on Harmonization and it exhibits satisfactory results for all parameters. The developed approach is capable of being used to conduct routine evaluations of telmisartan in bulk and marketed formulation.