Abstract

Microbiological quality assessment and antibiotic sensitivity testing are crucial components of ensuring public health safety, food quality, and effective antimicrobial therapy and provides a comprehensive overview of the methodologies, challenges, and recent advancements in microbiological quality assessment and antibiotic sensitivity testing. It covers various techniques employed in microbiological quality assessment, including culture-based methods, molecular techniques, and rapid detection methods. Additionally, it explores the principles and methodologies of antibiotic sensitivity testing, focusing on disk diffusion, broth microdilution, and automated systems. Furthermore, the article discusses emerging trends and technologies in the field, such as whole-genome sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and understanding these techniques and their applications is essential for researchers, healthcare professionals, and policymakers to combat microbial infections effectively and safeguard public health.

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