Exploring interactions between saprotrophic microbes and ectomycorrhizal fungi using a protein-tannin complex as an N source by red pine (Pinus resinosa).

Abstract

• Recent studies suggest that some plants may circumvent N mineralization carried out by saprotrophs because their ectomycorrhizal fungi have the capacity to hydrolyse protein. When complexed by tannins, however, proteins may be unavailable to some ectomycorrhizal fungi. • Here we tested the hypothesis that when protein-tannin complex is the N source, Pisolithus tinctorius will promote N uptake into red pine (Pinus resinosa) only in the presence of saprotrophs. • The model protein-tannin complex was stable at field pH. P.tinctorius could not obtain N from it, but saprotrophs could. Pre-treatment of the complex by saprotrophs did make its N available to ectomycorrhizal fungi. However, when the protein-tannin complex was the major N source, P.tinctorius increased shoot P but not N content, even in the presence of saprotrophs. • Interactions between saprotrophs and ectomycorrhizal fungi may be different for N and P because of immobilization of N by ectomycorrhizal fungi, or by the more rapid diffusion of ammonium than phosphate, rendering the absorptive surface area of ectomycorrhizal fungi superfluous for uptake of N but not for P.