Abstract
Landraces are valuable sources of genetic characteristics, which are of plant breeders’ interest to include in breeding programs for crop improvement. We assess the inter- and intra-genetic variability among 96 accessions representing three Hail tomato landrace using DNA-based marker sequence-related amplified polymorphism (SRAP). Seven SRAP primer combinations generated 55 alleles with a polymorphism of 100%, and an average of 7.86 polymorphic alleles per pair of primers. All primers showed an average of 0.68 polymorphic information content (PIC) value and discrimination power (DP) of 14.29. Principal coordinate analysis (PCoA) confirmed the clustering produced via the UPGMA similarity dendrogram allowed for the grouping of the 96 accessions according to its gene bank accession numbers and showed relatively good separation between landraces. A similarity value ranged from 0.04 to 1.0 among accessions of Hail 747, 0.05 to 1.0 in Hail 1072, and from 0.16 to 0.92 in Hail 548. These results showed the landraces harbor a wide range of genetic diversity at both inter and intra-variation levels. AMOVA showed that most of the genetic variation was because of differences within populations (87%). Tomato Hail landraces have well-differentiated genetic populations and admixtures, where Hail 747 formed their separate group, and both Hail 548 and Hail 1072 were admixed, and some accessions showed more diversity pattern. We have to take the SRAP technique’s effectiveness in the study of genetic variability among and within landraces into consideration in the tomato-breeding programs through marker-assessed selection.
Highlights
College of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia; Academic Editor: Mario A
The number of alleles scored per primer combination ranged from three for primer SRAP3 to 10 for primer SRAP5
The polymorphism (%), polymorphic information content (PIC), and discrimination power (DP) were higher than the results of several sequence-related amplified polymorphism (SRAP) marker research works [9,10,11,16,22], using other molecular markers, AFLP [23], ISSR [12,13,14,24], and RAPD markers [25,26]
Summary
College of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia; Academic Editor: Mario A. We assess the inter- and intra-genetic variability among 96 accessions representing three Hail tomato landrace using DNA-based marker sequence-related amplified polymorphism (SRAP). Tomato Hail landraces have welldifferentiated genetic populations and admixtures, where Hail 747 formed their separate group, and both Hail 548 and Hail 1072 were admixed, and some accessions showed more diversity pattern. Introduction with regard to jurisdictional claims in Tomato hybrids are the primary source of tomato cultivation These resources have narrow genetic bases because of several population bottlenecks in the natural, artificial selection and forms of founder events during the evolution and domestication of modern cultivars [1]. Farmers still grow tomato landraces on small farms because of the quality and particular demand of some consumers These landraces are valuable sources of genetic characteristics, which are of plant breeders’ interest to include in breeding programs for crop improvement. Conti et al [3] reported that Italian local tomato varieties exposed to water stress showed a valuable reservoir of genetic biodiversity with more water stress-resistance, which opens research toward understanding the mechanisms of response to drought in these local published maps and institutional affiliations
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