Abstract
Duck follicles enter different reproductive phases throughout life, and follicle gene expression patterns differ according to these phases. In particular, differentially expressed genes and related to development of follicle (mRNAs) play an important role to explore the key genes in this process; however, the expression profiles of these genes remain unclear. In this study, transcriptome sequencing was used to investigate the expression levels of duck ovarian genes, and comparative transcriptional analysis was carried out to identify differential genes, and cluster them into groups and function identification. The results showed differential expression of 593 coding genes between young and laying ducks, and of 518 coding genes between laying and old ducks. In further GO analysis, 35 genes from the comparison between old ducks and laying ducks have significant been changed involved in hormones related to follicle development. They include up-regulated genes StAR, CYP17, EPOX, 3β-HSD, CYP1B1 CYP19A1 and down-regulated genes SR-B1 in laying ducks hormone synthesis than old ducks. Among which EPOX is a key gene for time special highly expression during egg laying stage, and other key regulatory genes’ highly expression showed in young and laying stage, and lower expression showing with follicular development stopping. Therefore, EPOX is a key regulator for duck follicle development in laying period, its expression level increase 100 times higher than in youth and decrease 98% than stop laying period in duck life cycle.
Highlights
Maintenance of the physiological status of ovaries at different times requires serial specific genes and some biology molecular, such as regulation element function protein
The results of EXOP and StAR gene expression level verification were consistent with the RPKM results
The results of this study showed that steroidogenic synthesis pathway have same key differentially expressed genes (DEGs) in duck, but the expression mode of key DEGs are different, such as when the expression level of EPOX decreased from 100% of the reproductive stage to 2%, the expression of indirectly regulated gene was significantly reduced
Summary
Maintenance of the physiological status of ovaries at different times requires serial specific genes and some biology molecular, such as regulation element function protein. For egg-laying poultry, the ovary follicle is characterized by three life phases: the growth phase, laying phase, and maternity phase. The primordial follicle is assembled and prepared for egg-laying at sexual maturation, and remains in a quiescent state [1]. Exploring key genes related to development of follicle by RNA-seq in ducks. Research System CARS-43-2 Pro, to Lizhi Lu; Earmarked Fund for National Waterfowl-industry Technology Research System CARS-42-2 Pro, to Xiaolin Liu; and Zhejiang Major Scientific and Technological Project of Agricultural (livestock’s) Breeding 2016C02054-13 Pro, to Lizhi Lu
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