Exploring differentially expressed genes in Phaseolus vulgaris L. during BCMV infection

Abstract

Bean common mosaic virus (BCMV) is a significant pathogen that affects common bean, leading to substantial yield losses and reduced crop quality. To mitigate BCMV attacks, certain genes, including diacylglycerol kinases genes (PvDGKs), genes associated with defense and stress responses (PvGST, PvPAL,PvLOX, and PvPOD), as well as genes related to plant defense (PvPR1, PvPR2, and PvPR3) play an essential functional role in various stress responses in common bean. In this study, the expression levels of PvDGK1, PvDGK2, PvDGK3, PvDGK5a, PvDGK5b, PvDGK6, PvGST, PvPAL,PvLOX, PvPOD, PvPR1, PvPR2, and PvPR3 genes were investigated in the leaves of different common bean genotypes under BCMV infection conditions. Through quantitative real-time PCR analysis, we observed varying expression patterns for all these genes at different time points during viral infection. The tolerant genotype exhibited higher expression levels of all PvDGKs, PvGST, PvPAL, PvPOD, PvPR1, and PvPR2 genes compared to the susceptible genotype, with the PvPR1 gene showing the highest transcript levels. These findings provide the initial evidence of the potential roles of PvDGKs, PvGST, PvPAL,PvLOX, PvPOD, PvPR1, PvPR2, and PvPR3 in responding to the stress induced by BCMV in common bean. The results presented herein will serve as a valuable resource for guiding future breeding studies aimed at addressing BCMV-induced stress in common bean cultivation.