Explorative Proteomic Characterization of Platelet Rich Fibrin and Platelet Rich Fibrin Matrix and Their Potential Contribution in Repair and Regeneration – a Pilot Study

Abstract

Dental caries and pulpal diseases are serious global oral health care burden. Regenerative procedures are receiving increasing research attention in an attempt to restore lost tissue structure and function contributing to improved patient care. Many proteins, cytokines and growth factors are involved in tissue regeneration and act in orchestrated steps to restore tissue function. Platelet concentrates such as Platelet Rich Fibrin (PRF) and Platelet Rich Fibrin Matrix (PRFM) are blood derivatives rich in growth factors, cytokines, and signaling molecules and have evolved as a pioneering regenerative bioactive materials. PRF is rich in platelets and leucocytes with loose fibrin matrices, whereas PRFM a variant of PRF family is devoid of leucocytes. Though the main constituent of these concentrates is the fibrin scaffold, little is known about the proteins interacting in these and their role in the healing process. Identification of proteins in PRF and PRFM could deepen understanding of underlying molecular mechanisms in tissue regeneration, optimize formulation and dosage in regenerative therapies. It could enable us to further explore and unravel better therapeutic strategies to facilitate repair and regeneration, and improvise scope of translational research. Hence this study aimed to characterize the proteins enmeshed in PRF and PRFM scaffolds.