Explorative Data Analysis of In-Vitro Neuronal Network Behavior Based on an Unsupervised Learning Approach

Abstract

In the present chapter authors want to expose new insights in the field of Computational Neuroscience at regard to the study of neuronal networks grown in vitro. Such kind of analyses can exploit the availability of a huge amount of data thanks to the use of Multi Electrode Arrays (MEA), a multi-channel technology which allows capturing the activity of several different neuronal cells for long time recordings. Given the possibility of simultaneous targeting of various sites, neuroscientists are so applying such recent technology for various researches. The chapter begins by giving a brief presentation of MEA technology and of the data produced in output, punctuating some of the pros and cons of MEA recordings. Then we present an overview of the analytical techniques applied in order to extrapolate the hidden information from available data. Then we shall explain the approach we developed and applied on MEAs prepared in our cell culture laboratory, consisting of statistical methods capturing the main features of the spiking, in particular bursting, activity of various neuron, and performing data dimensionality reduction and clustering, in order to classify neurons according to their spiking properties having showed correlated features. Finally the chapter wants to furnish to neuroscientists an overview about the quantitative analysis of in-vitro spiking activity data recorded via MEA technology and to give an example of explorative analysis applied on MEA data. Such study is based on methods from Statistics and Machine Learning or Computer Science but at the same time strictly related to neurophysiological interpretations of the putative pharmacological manipulation of synaptic connections and mode of firing, with the final aim to extract new information and knowledge about neuronal networks behavior and organization.