Exploration Streptomyces sp. on the Soil of Several Vegetations in the Forest Zone Area of Alas Purwo Banyuwangi National Park and Tests of its Inhibitory on Escherichia coli O157:H7

Abstract

Problems related to antibiotic resistance are getting higher every time. Therefore, studies related to the exploration of new sources of antibiotics are very important to do. One source of antibiotics that has the potential to be utilized is derived from Streptomyces sp. This bacteria belongs to the Gram-positive bacteria of the Streptomycetaceae family which has the ability to produce enzyme and antibiotic is often found in natural soils. This study aims to explore the bacteria Streptomyces sp. in grassland vegetation and tropical rain forests in the jungle zone in the Alas Purwo National Park Area East Java Indonesia and tested its ability to inhibit the growth of Escherichia coli O157:H7. Sampling in this study was carried out in two different vegetation areas of the Alas Purwo National Park. The first vegetation is Sadengan grassland and the second is forest in the jungle zone. Soil samples were taken for each type of vegetation at five points. To isolate Streptomyces sp. used pour plate method with selective media YEMA (Yeast Extract Malt Agar). Bacterial isolates suspected as Streptomyces sp. and different from one another are separated to be re-isolated and identified macroscopically and microscopically Seung and Good fellow (2002). Twenty Streptomyces sp. isolates were successfully isolated from 2 vegetations. The highest inhibition was Streptomyces sp. O (2.725 cm) against Escherchia coli O157:H7, while the one with the lowest inhibition was Streptomyces sp .G (0.542 cm). In the future, after carrying out molecular tests, Streptomyces sp.O can be used as an alternative new antibiotics to combat pathogenic bacteria.