Exploration of the Microstructure and Rheological Properties of Sodium Alginate-Pectin-Whey Protein Isolate Stabilized Β-Carotene Emulsions: To Improve Stability and Achieve Gastrointestinal Sustained Release.

Haoxin Ye,Wenjun Fang,Min Huang,Qunfang Lei,Hujun Xie,Tingshuai Chen,Gerui Ren

doi:10.3390/foods10091991

Haoxin Ye, Wenjun Fang + Show 5 more

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https://doi.org/10.3390/foods10091991

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Abstract

Sodium alginate (SA)-pectin (PEC)-whey protein isolate (WPI) complexes were used as an emulsifier to prepare β-carotene emulsions, and the encapsulation efficiency for β-carotene was up to 93.08%. The confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) images showed that the SA-PEC-WPI emulsion had a compact network structure. The SA-PEC-WPI emulsion exhibited shear-thinning behavior and was in a semi-dilute or weak network state. The SA-PEC-WPI stabilized β-carotene emulsion had better thermal, physical and chemical stability. A small amount of β-carotene (19.46 ± 1.33%) was released from SA-PEC-WPI stabilized β-carotene emulsion in simulated gastric digestion, while a large amount of β-carotene (90.33 ± 1.58%) was released in simulated intestinal digestion. Fourier transform infrared (FTIR) experiments indicated that the formation of SA-PEC-WPI stabilized β-carotene emulsion was attributed to the electrostatic and hydrogen bonding interactions between WPI and SA or PEC, and the hydrophobic interactions between β-carotene and WPI. These results can facilitate the design of polysaccharide-protein stabilized emulsions with high encapsulation efficiency and stability for nutraceutical delivery in food and supplement products.

Highlights

IntroductionAs a vitamin A source, β-carotene is an effective plant medicine for dry eye [1]
Introduction βCarotene is a very important nutrient and pigment in human health
The results indicated that it was difficult to dissolve β-carotene in the citric acid-sodium citrate buffer, while the Sodium alginate (SA)-PEC-whey protein isolate (WPI) stabilized β-carotene emulsion was well dispersed in the buffer, indicating that the interaction between SA-PEC-WPI and β-carotene can promote the dissolution of β-carotene in the buffer to increase the EE of β-carotene

Summary

Introduction

As a vitamin A source, β-carotene is an effective plant medicine for dry eye [1]. It can reduce the incidence rate of macular disease [2] and can help prevent oral cancer [3]. The stability of β-carotene is low and can be degraded by photooxidation and thermal oxidation. The bioavailability of β-carotene directly from food is very low because its absorption effect in the gastrointestinal tract is not good [4]. Some strategies including emulsion, nanoparticle and liposome have been applied to improve the stability and bioavailability of β-carotene [5,6,7,8,9,10,11]

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