EXPLORATION OF THE ANTIBACTERIAL, ANTIOXIDANT AND ANTICANCER POTENTIAL OF THE SEED COAT EXTRACT OF MUNGBEAN (VIGNA RADIATA L. WILCZEK)

Abstract

Plant derived products are not just the part of our food but also it contains many phytochemicals that possess many medicinal properties to tackle various ailments. Studies on the phytochemicals in the food sources might lead to the discovery of more safe and reliable drugs. So keeping it in mind, present study aims on exploring the potential antibacterial, antioxidant and anticancer potential of the seed coat extract of Vigna radiata. Seeds being very rich source of easily digestible proteins are part of vegetarian diet. Preliminary study on phytochemicals showed the significant differences in the composition of the seed coats and seeds without seed coat. Methanolic extract of seed coat showed good antibacterial activity against both Gram positive and negative bacteria with zones of inhibition ranging from 25-28.5 mm in diameter at concentration of 10 mg/ml. The extract also exhibited good antioxidant potential when tested with two different methods that are DPPH (2,2-diphényl-1-picrylhydrazyl) radical scavenging activity and FRAP (Ferric Reducing Antioxidant Power Assay) method showing activity of 80% and 77% respectively at the concentration of 100 µg/ml. For anticancer activity checked by SRB assay against the human lung cancer cell line HOP-62, the extract at the concentration of 80 µg/ml showed 65% cell growth inhibition activity. The work concludes that seeds must be consumed with seed coat which has medicinal values due to rich antioxidant, antibacterial activity and anticancer potential thereby it could act as raw source of drugs for treating various ailments.