Abstract
Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) is a widespread plant with medicine value and has been used for treating various diseases, such as coughs, fevers, anemia, blood disorders and stomach pains. It had been reported that plant miRNAs might enter mammalian intestines and exert a special ingredient across the different species. However, the knowledge about miRNAs in N.cadamba is scarce. In this study, we first applied high-throughput sequencing to identify miRNAs in N.cadamba. Sequencing revealed a total of 11,285,965 raw reads in the small RNA library of N.cadamba leaves. By bioinformatics analysis, we identified a total of 192 miRNAs, including 167 conserved miRNAs and 25 novel miRNAs, which were distributed among 30 families. Next, we used miRanda to predict the targets of those miRNAs in human, 4030 target genes were predicted. Furthermore, Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that the identified miRNAs majored in binding, vibrio cholerae infection and insulin signaling, which were likely to provide valuable references for further understanding of medical functions of N. cadamba.
Highlights
The N. cadamba is a member of the tribe Naucleeae in the family Rubiaceae and distributed widely in South Asia and the south of China [28]
N. cadamba as a representative medicinal plants which is widely known as India Ayurvedic system of Medicine (IASM) [15] The studies of N. cadamba have mainly been focused on its medicinal values in the treatment of eye infections, antidiarrheal, skin diseases, dyspepsia and the stomatitis, coughs, fevers, anaemia, blood disorders and stomach pains [4, 26]
Further studies showed these plant microRNAs were absorbed from food; one that was relatively high in serum, miR168a, directly targeted low-density lipoprotein receptor adaptor protein 1
Summary
The N. cadamba is a member of the tribe Naucleeae in the family Rubiaceae and distributed widely in South Asia and the south of China [28]. MicroRNAs (miRNAs) are a class small non-coding RNAs, which are 18–24 nucleotide in length and inhibit gene expression by mRNA cleavage or translation repression in the 3′ untranslated region (3’UTR) [8]. Several years ago, plant microRNAs were discovered in sera and tissues of human and other animals [35]. Further studies showed these plant microRNAs were absorbed from food; one that was relatively high in serum, miR168a, directly targeted low-density lipoprotein receptor adaptor protein 1
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