Exploration of low-cost agro-industrial waste substrate for cellulase and xylanase production using Aspergillus heteromorphus

Somvir Bajar,Anita Singh,Narsi R Bishnoi

doi:10.1007/s13201-020-01236-w

Abstract

The current study was executed to explore the nutritive potential of low-cost agro-industrial waste substrates including anaerobically treated distillery spent wash (ADSW) and rice straw (RS) for enzyme production (cellulase and xylanase). The statistical model, Box–Behnken Design (BBD), was utilized to optimize the enzyme production through Aspergillus heteromorphus under liquid state bioconversion on examining the interactive interaction between substrate (ADSW), co-substrate (RS), temperature and initial pH. The BBD results revealed that the optimum levels of different factors for enzymes production were 6% ADSW, 3% RS, pH 5 and temperature 32.5 °C. The highest exoglucanase, xylanase and endoglucanase enzyme activities under optimum conditions were 6.3 IU/mL, 11.6 IU/mL and 8.1 IU/mL, respectively. The results confined that the agro-industrial waste substrate could be a cost-effective and eco-friendly alternative to produce value-added products like enzymes.

Highlights

The management of agro-industrial waste is very important as it poses a serious threat to the environment
Box–Behnken Design (BBD) was used to study the linear, quadratic and interactive effects of independent variables considered for the current study on cellulase and xylanase enzyme production by A. heteromorphus
The results revealed that the coefficient for the quadratic effects of anaerobically treated distillery spent wash (ADSW), rice straw (RS), initial pH, and temperature was highly significant for all responses

Summary

Introduction

The management of agro-industrial waste is very important as it poses a serious threat to the environment. A large amount of agro-industrial waste like rice straw, wheat straw, rice husk, bagasse, industrial effluents, sludge, etc., are produced annually, which create problem associated with disposal of these solid and liquid waste. The management of by-products is an augmented approach to achieve low carbon dioxide emissions (Weber and Stadlbauer 2017). The agricultural residues left after harvesting are either burned on-site (which creates air pollution and affects soil quality) or stored in the form of heaps in the field. Management of the agricultural waste is becoming one of the serious issues nowaday.

Methods

Results

Conclusion