Abstract
Cellulose is the most abundant organic polymer on Earth. Cellulose is an important structural component of the primary cell wall of green plants. Cellulose degrading bacteria from termite gut flora were isolated, screened and their characterization was studied in relation to cellulase activity. Out of the 16 isolates the three strains, showed higher production of cellulase using CMC and Blotting paper compared to filter paper as sole carbon source. CDB-W utilized CMC in higher level while CDB-Y utilized blotting paper in higher level. The maximum growth was recorded at pH 7.0 and at temperature 30°C. Among three isolates, the CDB- W strain showed higher enzyme activity as compared to other strains. Among carbon sources, maximum growth was observed in fructose amended MSM medium followed by Xylose, Glucose and Starch. Ammonium nitrate and potassium nitrate were good nitrogen sources for growth of CDB. The cellulolytic activities of these organisms may be utilized for various industrial and biotechnological applications for sustainable development.
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