Abstract
Abscisic acid (ABA) plays an important role in the regulation of plant adaptation, seed germination, and root development in plants. However, the mechanism of ABA regulation of root development is still poorly understood, especially through the miRNA-mediated pathway. Here, small RNA (sRNA)-seq and degradome-seq were used to analyze the miRNAs’ responsive to ABA in the stems and roots of P. euphratica, a model tree species for abiotic stress-resistance research. In total, 255 unique mature sequences, containing 154 known miRNAs and 101 novel miRNAs were identified, among which 33 miRNAs and 54 miRNAs were responsive to ABA in the roots and stems, respectively. Furthermore, the analysis of these miRNAs and their targets revealed a new hormone signaling crosstalk model of ABA regulation of root growth through miRNA-mediated pathways, such as peu-miR-n68 mediation of the crosstalk between ABA and the brassinosteroid (BR) signaling pathway and peu-miR477b mediation of the crosstalk between ABA and Gibberellic acid (GA) signaling. Taken together, our genome-wide analysis of the miRNAs provides a new insight into the mechanism of ABA regulation of root growth in Populus.
Highlights
Abscisic acid (ABA) plays a pivotal role in plant responses to biotic and abiotic stresses, such as pathogens, salinity, wounding, and water shortage [1]
ABA levels chang under different conditions and are modulated by the balance between ABA catabolism and biosynthesis [2], with 9-cis-epoxycarotenoid dioxygenase 3 (NCED3) being a vital enzyme for ABA biosynthesis [3] and ABA 8 -hydroxylases being a key enzyme in ABA catabolism [4]
The most remarkable development of the ABA signaling pathway is the discovery of the ABA receptor, PYR/PYL/RCAR, which functions at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting type 2C protein phosphatases (PP2Cs) [7,8]
Summary
Abscisic acid (ABA) plays a pivotal role in plant responses to biotic and abiotic stresses, such as pathogens, salinity, wounding, and water shortage [1]. As one of the most important phytohormones, the mechanisms of ABA signaling have been studied extensively for decades. An overwhelming number of studies regarding ABA signaling components that regulate seed germination and stomata have been completed [5,6]. The most remarkable development of the ABA signaling pathway is the discovery of the ABA receptor, PYR/PYL/RCAR, which functions at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting type 2C protein phosphatases (PP2Cs) [7,8]. ABA perception by RCARs/PYR1/PYLs plays an important role in the regulation of stomatal aperture, seed germination and vegetative and reproductive growth [9]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
