Exploration of 5-Azacytidine and Trichostatin A in the modulation of postharvest quality of 'Shine Muscat' grape berries

Abstract

Grapes have excellent commercial characteristics and high nutritional value, but postharvest storage inevitably reduces their quality. Previously, we studied the regulatory mechanisms of methylation for grape ripening. To further investigate the effects of epigenetic modifications on postharvest quality of grape berries, Transcriptome deep sequencing (RNA-seq) and metabolome sequencing were performed on 5-Azacytidine (5azaC) or Trichostatin A (TSA) treated grape berries. TSA delayed the depletion of soluble sugar and organic acid content in postharvest grapes compared to controls, while 5azaC had the opposite effect. In addition, TSA and 5azaC affected the content and composition of volatile organic compounds (VOCs) in the berries, of which the decrease in linalool content may be directly responsible for the reduction of muscat flavor in the berries. RNA-Seq analysis showed that 626 and 2253 differentially expressed genes (DEGs) were generated by 5azaC and TSA treatments, respectively. GO and KEGG enrichment analyses revealed significant differences in the biological pathways involved in the two collections of DEGs. Metabolomic analyses further clarified the marker metabolites induced by TSA and 5azaC related to fruit quality. In addition, the results of HDAC activity and transcript levels of acetylation-related genes suggest that VvHDA15 may acetylation modification affects the key regulators of postharvest fruit quality. This study not only provides abundant data information, but also offers new insights into the regulation of fruit quality by epimodification.