Exploration its Antioxidant and Immunogenic potential of protein: Calendula officinalis and Dianthus chinensis

Abstract

Aim and objective: Pollen is reported in flowers and leaves of various medicinal plants and showed its beneficial effect on human health. The objective of our study is to evaluate the antioxidant effect of flowers (Calendula officinalis and Dianthus chinensis) especially pollen protein and also determining its immunogenic effect against specific protein (typhoid vaccine) antigen in human whole blood samples. Methods: In this study, analytical studies were conducted for estimating the protein content using Lowry test and qualitatively determined through high performance thin layer chromatography (HPLC). In addition, immunological studies were also conducted using pollen protein for evaluating its antioxidant potential (using DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical assay) and determined its immunogenic effect using typhoid vaccine antigen and determining its hemolytic effect in human whole blood samples. Results: This work demonstrated that pollen protein content (determined through lowry test) at higher concentrations exhibited a significant declining in antigen specific T cell proliferation using optimized dose of typhoid vaccine antigen in human whole blood samples. In addition, pollen protein showed antioxidant property as well and does not show any hemolytic effect. Conclusion: The current study showed that pollen protein from Calendula officinalis and Dianthus chinensis possesses antioxidant property and less immunogenic in terms of declining in antigen specific T cell proliferation rate.