Abstract
Objective: A rabbit model of schistosoma granuloma was established by injecting Schistosoma japonicum eggs via carotid artery after opening blood-brain barrier with mannitol. Methods: Rabbits in the experimental group were injected with 1 ml of Schistosoma japonicum egg suspension after injecting mannitol into carotid for 20 min to establish a rabbit model; The rabbits in the negative control group were injected with mannitol through carotid artery for 20 min, followed by 1 ml normal saline injection; rabbits in the positive control group were injected with 1 ml suspension of Schistosoma japonicum eggs after coning cranial. The clinical manifestations of three groups of animals were observed after surgery, and brain tissue was taken for pathological examination 30 days later. Results: Brain histopathological examination: one rabbit in the experimental group showed inflammatory changes and no granuloma; no granuloma and inflammatory changes were observed in the negative control group; in the positive control group, granulomatous changes were observed in two rabbits and fibrillary glia astrocytosis in eight rabbits. Conclusion: Further studies are needed to establish an animal model by injecting eggs into the carotid artery and the method of injecting schistosoma egg suspension into brain tissue after skull drilling has a high success rate
Highlights
Brain schistosomiasis was first reported by Yamagiwa in 1889, and came to widespread attention after the US invaded the Philippines in 1944 [1]
A rabbit model of schistosoma granuloma was established by injecting Schistosoma japonicum eggs via carotid artery after opening blood-brain barrier with mannitol
Rabbits in the experimental group were injected with 1 ml of Schistosoma japonicum egg suspension after injecting mannitol into carotid for 20 min to establish a rabbit model; The rabbits in the negative control group were injected with mannitol through carotid artery for 20 min, followed by 1 ml normal saline injection; rabbits in the positive control group were injected with 1 ml suspension of Schistosoma japonicum eggs after coning cranial
Summary
Brain schistosomiasis was first reported by Yamagiwa in 1889, and came to widespread attention after the US invaded the Philippines in 1944 [1]. Some scholars opened the blood-brain barrier by radiotherapy and injected schistosoma eggs through the carotid artery; imaging studies showed successful modeling [5]. Injection after skull drilling directly destroys the blood-brain barrier, and radiotherapy has a certain destructive effect, and can reduce animal immunity. To reduce these disadvantages, in this study, the animal model of brain schistosoma granuloma was further explored by injecting the egg suspension after opening the blood-brain barrier by injecting mannitol into carotid artery
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