Abstract
Spermatozoa separated electrophorectically in 6 different buffers were inseminated into luteinizing hormone (LH)-primed rabbit does. Sex ratios of rabbits from anodic and cathodic sperm were significantly different from expected sex ratio when phosphate Lucks phosphate and Afif buffers were used (p less than .005). Average litter size and fertility rate were: 1)phosphate 4.1 and 75%; 2)Lucks phosphate 4.3 and 55%; and 3)Afif 4.7 and 75%. These mediums averaged 79% males with anodic sperm 38% with cathodic and 47% with both anode stopcock and cathode stopcock sperm. Afif buffer gave the most favorable results. Second third and fourth experiments did not substantiate the highly significant results of the first experiment. It is concluded that the theory of separation of X and Y chromosome-bearing spermatozoa by electric charge cannot by accepted or rejected with certainty.
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