Abstract
Pythium spp. were successfully isolated directly from sugar beet soils by the method of Ricci et al. (1976). The isolates obtained were divided in to four groups on the basis of morphological and growth habit characters. The groups showed large differences in in vitro pathogenicity. The importance of some factors affecting the estimation of Pythium propagule density is studied and discussed.
Highlights
Scientists have developed a diversity of techniques for the isolation of Pythium spp. from soil
The polyene antibiotic pimaricin has been widely used to suppress fungi other than Pythium spp. in selective media (Eckert& Tsao 1960, Sing & Mitchell 1961, Hine & Luna 1963, Mercetich& Fogle 1969)
This paper presents some preliminary experiments on direct isolation of Pythium spp
Summary
Scientists have developed a diversity of techniques for the isolation of Pythium spp. from soil. These techniques are based on two principles (Bouhot 1979): 1. 2. Incorporation of soil samples into an artificial selective medium, allowing each propagule of the parasite to develop into a colony. Robertson (1975) developed a paper disc technique for the recovery of Pythium spp. from soil or water. Numerous selective media have been reported for the isolation of Pythium spp. from soil. The polyene antibiotic pimaricin has been widely used to suppress fungi other than Pythium spp. in selective media (Eckert& Tsao 1960, Sing & Mitchell 1961, Hine & Luna 1963, Mercetich& Fogle 1969).
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