Abstract
A simple device capable of measuring almost any reactant in an enzymecatalyzed reaction is created when an enzyme is immobilized onto one thermal sensor of a differential thermometer. Experiments are described in which two thermistors, one bare and one coated with immobilized enzyme, are immersed in a well-stirred solution. The response of this device to increases in glucoseATP concentration was observed using hexokinase (ATP: d-hexose 6-phosphotransferase, EC 2.7.1.1), and to increases in glucose concentration using glucose oxidase (β- d-glucose:oxygen 1-oxidoreductase, EC 1.1.3.4). A simple model is presented whose predictions are in reasonable agreement with the experimental results.
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