Experimental use of fibrin glue derived from snake venom in non-pregnant canine uterus

J.R.E Moraes,F.R Moraes,A.C Camplesi,P.H.A Correia

doi:10.1590/s1678-91992004000200003

Abstract

This study evaluated the use of fibrin glue derived from snake venom in the healing process after canine hysterorrhaphy. Three groups of four animals were submitted to uterine hysterotomy followed by wound closure. In Group 1, double-layer suture was used, the first with Schimieden pattern, the second with Cushing pattern; in Group 2, only fibrin glue; and in Group 3, the same as for Group 1 but with fibrin glue as suture reinforcement. Results indicated that fibrin glue produced less inflammation in the exudative phase, and exacerbated deposition of connective tissue and angiogenesis in the proliferative and maturation phases of the healing process, favoring its evolution.

Highlights

Fibrin adhesives derived from snake venom are originally composed of plasma protein and snake venom (Crotalus durissus terrificus), imitating the last steps of the coagulation cascade, with the formation of a fibrin network [6]
This study evaluated the use of fibrin glue derived from snake venom in the healing process after canine hysterorrhaphy
Results indicated that fibrin glue produced less inflammation in the exudative phase, and exacerbated deposition of connective tissue and angiogenesis in the proliferative and maturation phases of the healing process, favoring its evolution

Summary

Introduction

Fibrin adhesives derived from snake venom are originally composed of plasma protein and snake venom (Crotalus durissus terrificus), imitating the last steps of the coagulation cascade, with the formation of a fibrin network [6]. This, in the presence of calcium and factor XIII is converted into a polymer with adhesive properties [13]. This adhesive, used in human skin grafts, shows total adhesion in 71.4% of patients and partial adhesion in 28.6%, which makes it a valuable alternative in skin surgery [11]. The results have shown that fibrin glue is to apply, requires short postoperative monitoring, and presents fast, good-quality skin healing [10]. In a similar model comparing conventional epineural suturing with fibrin glue derived from snake venom on sciatic nerve coaptation in Wistar rats, both techniques showed similar results, but fibrin glue presented better regeneration [14]

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