Abstract
Previous studies stated that TGF-β is a pleiotropic cytokine, abundant in mammalian intestine, regulates multiple cellular functions as a suppressor of the immune response, cell proliferation, and oncogenesis. Studyaims: Here, we investigated the value of TGF-β as a diagnostic marker of ulcerative colitis disease (UC) in experimental UC model. Materials& Methods: Acute & chronic UC was induced in mice by using (3% and 1.5% respectively) DSS in drinking water. The induction of UC was confirmed by histopathological examination. Proteins and mRNA expression of both TGF-β and TNF-α were analyzed by western blot and real time PCR respectively. Complete blood count (CBC), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR). Results: A marked elevation occurred in proteins and mRNA expression of both TGF-β and TNF-α in UC tissues in acute and chronic groups vs. healthy group. Furthermore, significant elevation in both CRP and ESR of diseased groups was recorded in comparison to normal group . Blood count revealed marked leukocytosis, thrombocytosis and marked decrease in red blood cell counts with severe anemia in both acute and chronic UC groups compared to healthy control group . Conclusion: Increased levels of TGF-β protein and mRNA expression in UC disease might have a diagnostic value side by side with inflammatorybiomarkers (TNF-α, CRP & ESR), leukocytosis, thrombocytosis, bloody diarrhea and histopathological changes.
Highlights
Ulcerative colitis (UC) is a type of inflammatory bowel diseases (IBD) characterized by inflammation typically bounded to colon with continuous lesions and damage the mucosal layer only (Satsangi et al, 2006, Gray et al, 2010)
Effects of ulcerative colitis disease (UC) induced by dextran sodium Sulphate (DSS) on TGF-β and TNF-α protein expression: We found marked elevation of TGF-β and TNF-α proteins expression vs. β-actin protein in colon tissue of DSS treated mice in acute and chronic group compared to healthy control group (Figure1 a & b)
Effects of UC induced by DSS on TGF-β and TNF-α mRNA expression: Our results revealed significant increase in mRNA expression of both TGF-β and TNF-α in both diseased groups vs. normal control group (Figure2 a & b)
Summary
Ulcerative colitis (UC) is a type of inflammatory bowel diseases (IBD) characterized by inflammation typically bounded to colon with continuous lesions and damage the mucosal layer only (Satsangi et al, 2006, Gray et al, 2010). Study aims: Here, we investigated the value of TGF-β as a diagnostic marker of ulcerative colitis disease (UC) in experimental UC model. Proteins and mRNA expression of both TGF-β and TNF-α were analyzed by western blot and real time PCR respectively. Results: A marked elevation occurred in proteins and mRNA expression of both TGF-β and TNF-α in UC tissues in acute and chronic groups vs healthy group. Blood count revealed marked leukocytosis, thrombocytosis and marked decrease in red blood cell counts with severe anemia in both acute and chronic UC groups compared to healthy control group. Conclusion: Increased levels of TGF-β protein and mRNA expression in UC disease might have a diagnostic value side by side with inflammatory biomarkers (TNF-α, CRP & ESR), leukocytosis, thrombocytosis, bloody diarrhea and histopathological changes
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