Experimental tumor growth of canine osteosarcoma cell line on chick embryo chorioallantoic membrane (in vivo studies)

Magdalena Walewska,Anna Małek,Katarzyna Zabielska-Koczywąs,Artur Żbikowski,Anna Wojtalewicz,Agata Wojtkowska,Roman Lechowski,Izabella Dolka

doi:10.1186/s13028-017-0298-8

Magdalena Walewska, Anna Małek + Show 6 more

Open Access

https://doi.org/10.1186/s13028-017-0298-8

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Abstract

The chick embryo chorioallantoic membrane (CAM) model is extensively used in human medicine in preclinical oncological studies. The CAM model has several advantages: low cost, simple experimental approach, time saving and following “3R principles”. Research has shown that the human osteosarcoma cell lines U2OS, MMNG-HOS, and SAOS can form tumors on the CAM. In veterinary medicine, this has been described only for feline fibrosarcomas, feline mammary carcinomas and canine osteosarcomas. However, in case of canine osteosarcomas, it has been shown that only non-adherent osteosarcoma stem cells isolated from KTOSA5 and CSKOS cell lines have the ability to form microtumors on the CAM after an incubation period of 5 days, in contrast to adherent KTOSA5 and CSKOS cells. In the presented study, we have proven that the commercial adherent canine osteosarcoma cell line (D-17) can form vascularized tumors on the CAM after the incubation period of 10 days.

Highlights

Osteosarcoma is the most common malignant bone tumor in children and dogs, the disease occurs in dogs approximately 10 times more frequently than in humans
The chick embryo chorioallantoic membrane (CAM) model is extensively used in preclinical oncological studies [1,2,3,4,5,6,7,8,9,10]
Histopathological examination and grading were performed according to a system adopted from human medicine and used in previous studies of canine subcutaneous soft tissue sarcomas, in this case for soft-tissue osteosarcomas [18]

Summary

Introduction

Osteosarcoma is the most common malignant bone tumor in children and dogs, the disease occurs in dogs approximately 10 times more frequently than in humans. The chick embryo chorioallantoic membrane (CAM) model is extensively used in preclinical oncological studies [1,2,3,4,5,6,7,8,9,10]. Growth of various human tumors (Burkitt lymphoma, ovarian cancer, glioblastoma multiforme, head and neck squamous cell carcinoma) was successfully achieved using the CAM model [6, 11,12,13].

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