Experimental Tobacco Carcinogenesis

Abstract

Publisher Summary The primary purpose of laboratory investigations in the field of tobacco carcinogenesis is to determine if various tobacco and tobacco smoke products might prove to be carcinogenic to different animal species and tissues, and to determine the components responsible for this activity. Once such components are identified, their removal or reduction is investigated. Reviewing the biological and chemical studies involving tumorigenic and cilia-toxic activity of tobacco products, fractions and single components, the chapter presents a discussion of pertinent analytical techniques employed, of different biological procedures utilized, of precursors that give rise to certain components, and of some fundamental aspects. The characteristics of tobacco and tobacco smoke such as tobacco products, physical state of tobacco smoke, smoking machines and smoking techniques, preparation of tobacco smoke, tobacco extracts, and combustion and smoke temperatures have also been discussed in the chapter. Biological tests for tumorigenic activity are tobacco smoke condensate, tobacco extracts, tobacco smoke, tobacco smoke condensate fractions, and cilia-toxic components. Components of tobacco and tobacco smoke listed in this chapter are considered to possess tumorigenic and cilia-toxic activity. Reduction of tumorigenic activity can be achieved by reduction of total smoke condensate, reduction of tumorigenic agents, and reduction of cilia-toxic agents. The chapter also provides an interpretation of experimental findings that include inhalation studies, statistical considerations, future studies, and relation to human data.