Experimental substantiation of the use of hydroxyapatite — tricalcium phosphate bioceramics for replacing bone defects after tumor removal

Abstract

Recently, bioactive ceramics based on hydroxyapatite (HAP) and tricalcium phosphate (TCP) have been preferred as implants in bone engineering. To study bone regeneration under conditions of filling metaphyseal defects with the original HAP-TCP composition. The experiment was carried out on inbred rats, the observation period was 2, 4, 8weeks. Morphological studies were carried out using light and electron microscopy. In the dynamics of observation at 2, 4and 8weeks, a gradual arrangement of osteoclasts and osteoblasts on the surface of HAP-TCP was recorded, which indicates its high biocompatibility with bone tissue. During the experiment, the processes of resorption of implants, mineralization, proliferation of cellular elements of collagen and osteogenesis (osteoblasts and osteoclasts) and the formation of mature bone tissue were recorded. Experimental studies of plastic bone defects proved the presence of the osteoinductive and osteointegrative effect of HAP-TCP composition, which contributes to a more dynamic uncomplicated course of reparative osteogenesis.