Abstract
Objective To investigate the intervention effect and mechanism of Aconite and Angelica sinensis on myocardial ischemia rats with Yang deficiency and blood stasis. Methods SPF-class SD rats were randomly divided into low-dose and high-dose groups. Each group was divided into control group, model group, and drug-administered group (FZ, DG, FG; 1 : 0.5, 1 : 1, 1 : 2). A rat model was prepared by intraperitoneal injection of hydrocortisone and isoproterenol plus cold stimulation. Each group was given corresponding decoction or distilled water for 14 days. The behavioral changes of rats in each group were observed. The morphological changes of rats cardiomyocytes were observed by HE staining. The average optical density (MOD value) and percentage of positive cells of Bcl-2, Bax, and Akt were determined by immunohistochemical staining method, and PEIs were calculated. Western blot and RT-PCR were used to determine the expression of PI3K, Caspase-3, Akt protein, and gene expression. Results The compatibility of Aconite and Angelica sinensis improved the morphology of rat cardiomyocytes, increased the PEI values of Akt and Bcl-2 protein, and decreased the PEI values of Bax protein (P < 0.01). The compatibility reduced the expression of Caspase-3 protein of rat myocardium and increased the protein expression of p-Akt, PI3K, and p-PI3K (P < 0.01). The compatibility also significantly reduced the expression of Caspase-3 mRNA and increased the expression of PI3K mRNA and Akt mRNA (P < 0.05 or P < 0.01), and the effect of high-dose FG (1 : 2) group is the best. Conclusions The method of preparing a rat model of myocardial ischemia with Yang deficiency and blood stasis was feasible. The compatibility of Aconite and Angelica sinensis reduced myocardial fibrosis and inflammatory reaction, protected ischemic cardiomyocytes, and reduced myocardial injury, whose mechanism may be related to the regulation of PI3K/Akt pathway. The compatible group had better intervention effects than Aconite or Angelica sinensis alone. The best one was high-dose FG (1 : 2).
Highlights
Myocardial ischemia (MI) is more common in coronary heart disease (CHD), which refers to heart disease caused by myocardial ischemia or hypoxia caused by coronary atherosclerosis and functional changes; it is a common disease with extremely high mortality, which belongs to the category of “thoracic,” “heartache,” and “syncope” of Chinese medicine
According to the results of modern pharmacological research, Chinese medicine Aconite has the functions of strengthening heart, boosting blood pressure, lowering blood sugar, and regulating nerve disorder and immune system, in addition to its antimyocardial ischemia, antithrombus, antiarrhythmia, antishock, antiaging, antitumor, and analgesic effects [1, 2]
FZ FG (1: 0.5) FG (1: 1) FG (1: 2) DG (c) compatibility groups of Aconite and Angelica sinensis, we found that the expression levels of Caspase-3 mRNA and Akt mRNA in FG (1 : 2) group were better than those in FG (1 : 1) and FG (1 : 0.5), while the expression of PI3K mRNA was not significantly different (P < 0.05)
Summary
Myocardial ischemia (MI) is more common in coronary heart disease (CHD), which refers to heart disease caused by myocardial ischemia or hypoxia caused by coronary atherosclerosis and functional changes; it is a common disease with extremely high mortality, which belongs to the category of “thoracic,” “heartache,” and “syncope” of Chinese medicine. According to the results of modern pharmacological research, Chinese medicine Aconite has the functions of strengthening heart, boosting blood pressure, lowering blood sugar, and regulating nerve disorder and immune system, in addition to its antimyocardial ischemia, antithrombus, antiarrhythmia, antishock, antiaging, antitumor, and analgesic effects [1, 2]. Angelica sinensis ferulic acid can significantly protect myocardial cells from hypoxia and reoxygenation injury by increasing NO production, inhibiting platelet aggregation and proliferation, and improving myocardial cell survival rate; Angelica sinensis and its ferulic acid can reduce cerebral infarction area in MCAO model rats by improving neurological deficit score, blood flow, and SOD activity [6, 7]
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