Abstract
目的研究特异性T细胞疫苗接种对大鼠肝移植后排斥反应的影响.方法利用LOU/CN大鼠的脾细胞免疫CHN大鼠,取后者脾细胞活化制备T细胞疫苗,对肝移植受者CHN大鼠进行免疫.测定T细胞疫苗接种与未接种组的混合淋巴细胞培养cpm值.采用三袖套法进行大鼠原位肝移植.按移植前后处理方法不同分为3组:(1)T细胞疫苗接种组:用T细胞疫苗接种后7～9d的CHN大鼠进行原位肝移植;(2)环孢素A(CsA)组:未接种T细胞疫苗的CHN大鼠进行原位肝移植后,用CsA10mg*kg-1*d-1进行免疫抑制治疗;(3)阴性对照组:未接种T细胞疫苗的CHN大鼠进行原位肝移植后,用生理盐水作对照.分别测定各组的存活时间、微量细胞毒反应.结果 T细胞疫苗接种组与未接种组外周血淋巴细胞cpm值分别为:9 804±1 182和22 386±2 117,二者比较,差异有显著性(P<0.01).异品系大鼠肝移植后,T细胞疫苗接种组存活时间为(17.1±2.8)d,CsA组为(14.2±3.1)d,阴性对照组为(11.6±2.4)d.三组比较,(P<0.01).微量细胞毒测定死细胞百分率:T细胞疫苗接种组为(26.4±9.3)%,CsA组为(43.7±10.4)%,阴性对照组为(62.4±8.1)%.三组比较,差异有显著性(P＜0.01).结论 T细胞疫苗接种能显著延长异品系大鼠肝移植的存活期,有显著的抗排斥反应作用.
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