Abstract
Objective To observe the effects of shikonin on the proliferation of lung adenocarcinoma cells H1975, and explore its possible anticancer mechanism. Methods The cytostatic effects of shikonin on H1975 cells were evaluated by MTT assay and the cell morphology was observed under the microscope.The effects of shikonin on apoptosis and cycle of H1975 cells were determined by flow cytometry (FCM). The expressions of epidermal growth factor receptor (EGFR) signal pathway related proteins and proteins of Bcl-2 and PARP in H1975 cells were detected by Western blot. Results MTT assay showed that shikonin could potently inhibit the growth of H1975 cells.FCM result showed that the apoptotic rate was significantly high and the blocking cell was in the S phase.The protein expressions of EGFR, ERK, AKT, p-EGFR, p-ERK, p-AKT, Bcl-2 in H1975 cells could be down-regulated, and expression of PARP protein could be markedly sheared by shikonin. Conclusions Shikonin can significantly inhibit H1975 cell proliferation and promote tumor cell apoptosis, which may be related to regulating the protein expression of Bcl-2, PARP and protein expressions in EGFR signal pathway. Key words: Shikonin; Non-small cell lung cancer; H1975; Anticancer mechanisms
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