Experimental study of resveratrol targeting pericytes against glioma

Abstract

Objective To investigate the effects of resveratrol (RES) on the angiogenesis of glioma cells. Methods U87 cells were used to establish a human orthotopic transplantation model of human glioma nude mice. They were randomly divided into RES drug treatment group and sodium carboxymethyl cellulose (CMC) solvent control group, 18 cases in each group. To observe the survival status of tumor-bearing nude mice, and to map the survival curve, using cell surface molecule CD34 (CD34), α-smooth muscle actin (α-SMA) Tumor microvascular endothelial cells and pericytes were labeled, and glioma microvessel density (MVD) and microvascular pericyte cell density (MPND) were measured, and orthotopic transplantation was observed by transmission electron microscopy. Ultrastructural changes of peripheral cells in glioma microvessels. Results The survival time of the RES group was significantly longer than that of the vehicle control group [(71.67±20.35), (53.41±11.62) d, P<0.05]. The MPND of the RES group was higher than that of the control group (16.12±1.60, 14.40±0.95, P<0.05). The MVD of the control group was higher than that of the treatment group (34.00±2.70, 25.43±1.54, P<0.05). The results of transmission electron microscopy showed that the body density (Vv) of the control group was higher than that of the treatment group (37.70±3.03, 22.00±1.68, P<0.05), and the control group had higher specific membrane surface (δm) than the treatment group (43.75±1.19, 40.10±2.26, P<0.05), the specific surface area (δ) of the treatment group was higher than that of the control group, (2.41±0.50, 1.20±0.60, P<0.05). Conclusion RES can increase the coverage of microvascular pericytes and disturb the perivascular cells of the tumor. Cell interactions inhibit angiogenesis in gliomas and produce anti-glioma effects. Key words: Resveratrol; Glioma; Angiogenesis; Pericytes; Ultrastructure