Experimental study of ASCs combined with POC-PLA patch for the reconstruction of full-thickness chest wall defects.

Yuanzheng Zhang,Chao Yang,Hao Fan,Haiying Dai,Shuo Fang,Ying Wang,Lei Zhu,Jiezhi Dai,Peipei Zhang,Weiya Tang,Yongjie Fan,Xin Xing

doi:10.1371/journal.pone.0182971

Abstract

To explore the repairing effect of combination of adipose stem cells (ASCs) and composite scaffolds on CWR, the electrospun Poly 1, 8-octanediol-co-citric acid (POC)-poly-L-lactide acid (PLA) composite scaffolds were prepared, followed by in vitro and in vivo biocompatibility evaluation of the scaffolds. Afterwards, ASCs were seeded on POC-PLA to construct the POC-PLA-ASCs scaffolds, and the POC-PLA, POC-PLA-ASCs, and traditional materials expanded polytetrafluoroethylene (ePTFE) were adopt for CWR in New Zealand white (NZW) rabbit models. As results, the POC-PLA-ASCs patches possessed good biocompatibility as the high proliferation ability of cells surrounding the patches. Rabbits in POC-PLA-ASCs groups showed better pulmonary function, less pleural adhesion, higher degradation rate and more neovascularization when compared with that in other two groups. The results of western blot indicated that POC-PLA-ASCs patches accelerated the expression of VEGF and Collagen I in rabbit models. From the above, our present study demonstrated that POC-PLA material was applied for CWR successfully, and ASCs seeded on the sheets could improve the pleural adhesions and promote the reparation of chest wall defects.

Highlights

Intact thoracic wall plays an important role in breathing movement and protects the indispensable content
The results showed that the positive expression rates of CD29, CD73, CD90 and CD105 were all higher than 90%, while CD31, CD34, CD44 and CD45 showed negative results (Fig 3b), which indicated the higher purity of adipose stem cells (ASCs) we isolated
Chung EJ et al fabricated a biodegradable and synthetic tri-component graft consisting of POC, hydroxyapatite nanocomposites (HA) and poly (L-lactide) (PLL), and revealed its good performance in a rabbit ligament reconstruction animal model [23]

Summary

Introduction

Intact thoracic wall plays an important role in breathing movement and protects the indispensable content. The full-thickness defects of the chest well commonly caused by tumor resection, trauma, infection, burn, and radiation [1,2]. Flaps have been developed for soft tissue reconstruction depend on the location on the chest wall, size of the defects, arc of rotation of the flap, and availability of recipient vessels. Bony reconstruction is performed with mesh or mesh combining with rib substitutes according to the number of defect ribs [3].

Methods

Results

Conclusion